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KMID : 0378019740170070879
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New Medical Journal
1974 Volume.17 No. 7 p.879 ~ p.885
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Experimental Studies on the Bacterial Growth in the Human Urine(Ⅱ)
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Abstract
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Previous observation showed that the mode of bacterial multiplication in the human urine varies depending upon the bacterial species, environmental temperature, and urinary pH and also indicated some difference between the multiplication pattern in male and female urines. Because of possibility of clinical implications, these findings seemed to warrant further investigations.
Twenty mid-stream urines were collected from healthy individuals of 10 male and 10 female. The urinalysis showed the biochemical and cytological findings of these urines within normal range. The urines were divided into 4 groups comprising 5 male or female urines each and the urines of same group were pooled. After adjusting the pooled urines to pH 5.5 or 6.5 with 1/10 N HC1 or 1 N NaOH solution, the urines were centrifuged at 5,000 r. p. m. for 30 minutes. In to the supernatants 18 hours broth culture of Staph. aureus or Es. coli, washed and resuspended in the saline, were inoculated. After 1,3,5, and 7 hours of aerobic incubation at 37°C., viable units in the urines were enumerated by serial pour-plate method.
Results of the observation were summarized as follows:
1. Viable units of Es. coli in the human urine, compared to those of Staph. aureus, increased more rapidly after shorter lag phase.
2. Multiplication of Staph. aureus in the urine was noticeably more rapid at pH 6.5 than at 5.5; while the difference in multiplication of Es. coli was less obvious.
3. Both bacterial species appeared to increase more rapidly, with varied degree depending on the urinary pH and organisms, in the female urine than in the male urine.
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